Whole genome based analyses are becoming increasingly important in biological research, spanning, but not limited to, evolutionary, medical, and conservation contexts. Genome assembly, an initial step in genomic analyses, is a rapidly developing area of research, and so staying up to date with its current state can be challenging. Furthermore, it can be difficult to understand for researchers new to the field. This workshop is targeted towards researchers having anywhere from no background up to advanced knowledge of genome assembly. It will function as a roadmap from designing genome sequencing projects all the way to obtaining a “final”genome assembly, with some brief discussion of downstream analyses. On the first afternoon, I will start very basic by covering pre-planning and laboratory topics such as the different sequencing technologies available, and how to decide on which sequencing platform and library preparation method to use. On the second afternoon, I will outline the different steps needed to process the raw sequencing data, as well as the different assembly, quality assessment and improvement methods. To make the workshop more user-friendly, I will discuss popular tools employed at the different steps.